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prime script reverse transcription reagent kit  (TaKaRa)


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    Structured Review

    TaKaRa prime script reverse transcription reagent kit
    Prime Script Reverse Transcription Reagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 88241 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prime+script+reverse+transcription+reagent+kit/PrimeScript+RT+Reagent+Kit/pm41211829-316-10-16
    Average 99 stars, based on 88241 article reviews
    prime script reverse transcription reagent kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: SPI1 affects Aβ 1 - 42 -induced microglia-mediated neuroinflammation by transcriptionally regulating FOSL2 expression.
    Article Snippet: Background: Microglia-induced-neuroinflammation contributes to Alzheimer’s disease (AD) progression.. SPI1 has been implicated in neuroinflammation and neuronal function and participated in AD progression.. However, the underlying mechanism of SPI1 in microglia-induced neuroinflammation remains unclear.

    Synthesized:

    Article Title: SPI1 affects Aβ 1 - 42 -induced microglia-mediated neuroinflammation by transcriptionally regulating FOSL2 expression.
    Article Snippet: Background: Microglia-induced-neuroinflammation contributes to Alzheimer’s disease (AD) progression.. SPI1 has been implicated in neuroinflammation and neuronal function and participated in AD progression.. However, the underlying mechanism of SPI1 in microglia-induced neuroinflammation remains unclear.

    Article Title: Toll like receptor 2 mediated exacerbation of sepsis associated acute kidney injury by renal congestion in mice.
    Article Snippet: Total RNA was extracted from the kidneys and NRK-52E using TRIzol (Life Technologies, Carlsbad, CA) and DirectzolTM RNA MiniPrep (Zymo Research, Irvine, CA) according to the manufacturer’s protocol. .. Complementary DNA was synthesized using a Prime Script reverse transcription reagent kit (RR0471A Takara Bio Inc., Shiga, Japan). .. The real-time detection of PCR products was performed using KAPA SYBR FAST qPCR Master Mix (2×) (KK4602: Kapa Biosystems, Wilmington, MA) and a Thermal Cycler Dice Real Time System (Takara Bio Inc., Shiga, Japan).

    Article Title: Toll like receptor 2 mediated exacerbation of sepsis associated acute kidney injury by renal congestion in mice
    Article Snippet: Total RNA was extracted from the kidneys and NRK-52E using TRIzol (Life Technologies, Carlsbad, CA) and DirectzolTM RNA MiniPrep (Zymo Research, Irvine, CA) according to the manufacturer’s protocol. .. Complementary DNA was synthesized using a Prime Script reverse transcription reagent kit (RR0471A Takara Bio Inc., Shiga, Japan). .. The real-time detection of PCR products was performed using KAPA SYBR FAST qPCR Master Mix (2×) (KK4602: Kapa Biosystems, Wilmington, MA) and a Thermal Cycler Dice Real Time System (Takara Bio Inc., Shiga, Japan).

    Article Title: LncRNA SNHG1 alleviates myocardial ischaemia–reperfusion injury by regulating the miR‐137‐3p/KLF4/TRPV1 axis
    Article Snippet: The total RNA was extracted with TRIzol (Invitrogen, Carlsbad, USA). .. The cDNA was synthesized with the Prime Script Reverse Transcription Reagent Kit (TaKaRa, Shiga, Japan) and subjected to RT‐qPCR assay using the SYBR Premix Ex Taq II Kit (Takara, Shiga, Japan). ..

    Reverse Transcription:

    Article Title: SPI1 affects Aβ 1 - 42 -induced microglia-mediated neuroinflammation by transcriptionally regulating FOSL2 expression.
    Article Snippet: Background: Microglia-induced-neuroinflammation contributes to Alzheimer’s disease (AD) progression.. SPI1 has been implicated in neuroinflammation and neuronal function and participated in AD progression.. However, the underlying mechanism of SPI1 in microglia-induced neuroinflammation remains unclear.

    Article Title: Toll like receptor 2 mediated exacerbation of sepsis associated acute kidney injury by renal congestion in mice.
    Article Snippet: Total RNA was extracted from the kidneys and NRK-52E using TRIzol (Life Technologies, Carlsbad, CA) and DirectzolTM RNA MiniPrep (Zymo Research, Irvine, CA) according to the manufacturer’s protocol. .. Complementary DNA was synthesized using a Prime Script reverse transcription reagent kit (RR0471A Takara Bio Inc., Shiga, Japan). .. The real-time detection of PCR products was performed using KAPA SYBR FAST qPCR Master Mix (2×) (KK4602: Kapa Biosystems, Wilmington, MA) and a Thermal Cycler Dice Real Time System (Takara Bio Inc., Shiga, Japan).

    Article Title: Ultrahigh Cell Density 3D Bioprinting by Acoustic Fluids-mediated Stereolithography.
    Article Snippet: Dense cell populations and their complex structures are the essentials of physiologically functional solid organs, but their biomimetic construction is challenging.. Here, an ultrahigh cell density (UHCD) bioprinting system is presented for fabricating organ-analogous intricate architectures based on an acoustic fluids-mediated stereolithography (ASL) technique.. The ASL can achieve cell aggregation on a prepolymerized solid–liquid interface with densities comparable to native tissues through focused surface acoustic waves-induced vortices.

    Article Title: GADD45A is Essential for Granulosa Cells Differentiation and Ovarian Reserve in Human and Mice.
    Article Snippet: Total RNA was isolated with a Total RNA Kit (Omega Biotek, Norcross, USA) according to the manufacturers' protocol. .. Then, a Prime Script reverse transcription reagent kit (TaKaRa, Tokyo, Japan) was applied for cDNA synthesis. .. Real- time PCR was carried out using SYBR green sequence detection reagents (Takara Tokyo, Japan) in a 20- μL reaction nloaded from https://onlinelibrary.w iley.com /doi/10.1111/jcm m .70820 by C apes, W iley O nline L ibrary on [10/09/2025].

    Article Title: GADD45A is Essential for Granulosa Cells Differentiation and Ovarian Reserve in Human and Mice
    Article Snippet: Total RNA was isolated with a Total RNA Kit (Omega Bio‐tek, Norcross, USA) according to the manufacturers' protocol. .. Then, a Prime Script reverse transcription reagent kit (TaKaRa, Tokyo, Japan) was applied for cDNA synthesis. .. Real‐time PCR was carried out using SYBR green sequence detection reagents (Takara Tokyo, Japan) in a 20‐μL reaction which contains 1 μL of cDNA, 10 μL of SYBR green, 1 μL of 10 μM primer (each) and 7 μL RNAase‐free water.

    Article Title: Toll like receptor 2 mediated exacerbation of sepsis associated acute kidney injury by renal congestion in mice
    Article Snippet: Total RNA was extracted from the kidneys and NRK-52E using TRIzol (Life Technologies, Carlsbad, CA) and DirectzolTM RNA MiniPrep (Zymo Research, Irvine, CA) according to the manufacturer’s protocol. .. Complementary DNA was synthesized using a Prime Script reverse transcription reagent kit (RR0471A Takara Bio Inc., Shiga, Japan). .. The real-time detection of PCR products was performed using KAPA SYBR FAST qPCR Master Mix (2×) (KK4602: Kapa Biosystems, Wilmington, MA) and a Thermal Cycler Dice Real Time System (Takara Bio Inc., Shiga, Japan).

    Article Title: LncRNA SNHG1 alleviates myocardial ischaemia–reperfusion injury by regulating the miR‐137‐3p/KLF4/TRPV1 axis
    Article Snippet: The total RNA was extracted with TRIzol (Invitrogen, Carlsbad, USA). .. The cDNA was synthesized with the Prime Script Reverse Transcription Reagent Kit (TaKaRa, Shiga, Japan) and subjected to RT‐qPCR assay using the SYBR Premix Ex Taq II Kit (Takara, Shiga, Japan). ..

    Article Title: Curcumin Regulates Microglia Polarization to Alleviate Ischemic Stroke by Targeting microRNA-205-5p/Kruppel-Like Factor 2 (KLF2)/Activating Transcription Factor 2 (ATF2) Axis.
    Article Snippet: Ischemic stroke (IS) often causes fearful sequela, even death.. Curcumin was beneficial to IS, but its underlying molecular mechanism is unclear.. Mice were subjected to middle cerebral artery occlusion (MCAO) surgery, and BV2 cells were treated with oxygen–glucose deprivation/reoxygenation (OGD/R) induction to establish IS models in vivo and in vitro.

    Real-time Polymerase Chain Reaction:

    Article Title: Ultrahigh Cell Density 3D Bioprinting by Acoustic Fluids-mediated Stereolithography.
    Article Snippet: Dense cell populations and their complex structures are the essentials of physiologically functional solid organs, but their biomimetic construction is challenging.. Here, an ultrahigh cell density (UHCD) bioprinting system is presented for fabricating organ-analogous intricate architectures based on an acoustic fluids-mediated stereolithography (ASL) technique.. The ASL can achieve cell aggregation on a prepolymerized solid–liquid interface with densities comparable to native tissues through focused surface acoustic waves-induced vortices.

    cDNA Synthesis:

    Article Title: GADD45A is Essential for Granulosa Cells Differentiation and Ovarian Reserve in Human and Mice.
    Article Snippet: Total RNA was isolated with a Total RNA Kit (Omega Biotek, Norcross, USA) according to the manufacturers' protocol. .. Then, a Prime Script reverse transcription reagent kit (TaKaRa, Tokyo, Japan) was applied for cDNA synthesis. .. Real- time PCR was carried out using SYBR green sequence detection reagents (Takara Tokyo, Japan) in a 20- μL reaction nloaded from https://onlinelibrary.w iley.com /doi/10.1111/jcm m .70820 by C apes, W iley O nline L ibrary on [10/09/2025].

    Article Title: GADD45A is Essential for Granulosa Cells Differentiation and Ovarian Reserve in Human and Mice
    Article Snippet: Total RNA was isolated with a Total RNA Kit (Omega Bio‐tek, Norcross, USA) according to the manufacturers' protocol. .. Then, a Prime Script reverse transcription reagent kit (TaKaRa, Tokyo, Japan) was applied for cDNA synthesis. .. Real‐time PCR was carried out using SYBR green sequence detection reagents (Takara Tokyo, Japan) in a 20‐μL reaction which contains 1 μL of cDNA, 10 μL of SYBR green, 1 μL of 10 μM primer (each) and 7 μL RNAase‐free water.



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